問答環節 Q&A
問: ERT-PCR和其他檢測技術有什麼區別?
答: ERT-PCR的靈敏度至少比其他方法高十倍。它使用多一組引物來提高特異性,並使用多一輪擴增來提高靈敏度。
Q: What is the difference between ERT-PCR and other detection technologies?
A: ERT-PCR is at least ten times more sensitive than other methods. It uses an extra set of primers and an additional round of amplification of the target sequence to increase sensitivity and specificity.
問: ERT-PCR是世界上靈敏度最高的核酸檢測方法嗎?
答: ERT-PCR的靈敏度已經處於極限,其精準度可達至一條核酸鏈就可以檢測到。當然,我們仍然需要使用臨床樣本進行驗證。
Q: Is ERT-PCR the most sensitive nucleic acid detection method in the world?
A: The sensitivity of ERT-PCR has already reached the highest level of sensitivity, and requires only one copy of the target sequence in order to be detected. We will continue to use clinical samples for validation.
問: 目前的方法可以在一小時內完成,為什麼ERT-PCR測試需要3-4小時?成本是多少?
答: ERT-PCR需進行多一輪擴增以增加靈敏度,因此需要更多時間。針無兩頭利,我們需要犧牲時間以換取靈敏度。成本的方面,只考慮試劑費用,每個反應大約需要50港元。
Q: Current methods can be completed in one hour. Why does the ERT-PCR test take 3-4 hours? How much does it cost?
A: Compared to the current method ERT-PCR involves an additional round of amplification of the target sequence to increase sensitivity, so it takes more time. Needless to say, we need to sacrifice time in exchange for sensitivity. In terms of cost, considering only the cost of reagents, each reaction is about HK $ 50.
問: 為什麼ERT-PCR技術不在市場上?
答: ERT-PCR是一種非常高靈敏度的方法。通常情況下,常用的RT-PCR就足夠了,但目前疫情的情況,我們認為它是檢測方法的首選。
Q: Why is ERT-PCR technology not on the market?
A: ERT-PCR technology is patented by Hai Kang Life Corporation Limited, we are working on expanding its application in different fields. In general, the commonly used RT-PCR and other methods are sufficient for their particular purposes. However, in the current epidemic situation, we think it should be the first choice as a detection method.
問: 如果新型冠狀病毒出現變種,您們能否成功檢測?
答: 要視乎病毒出現變種的序列位置;我們今次針對新冠病毒檢測的基因為保守序列,即使出現變種仍有大機會保持相同序列,保留成功檢測的可能性。
Q: If the new coronavirus mutates, can you successfully detect it?
A: This is dependent on the location of the mutation. The materials used to target COVID-19 are designed from the conserved regions of the sequence which have a low chance of mutating. Therefore, if a new strain of coronavirus emerges, we are most likely able to detect the conserved sequences ensuring the integrity of the technique. Nevertheless, we are confident in any additional approaches needed for precise differentiation.
問: ERT-PCR技術檢測如何操作和使用?
答: 我們準備了操作手册及視頻指導,希望為您提供更多幫助。
視頻指導:
YouTube: https://www.youtube.com/watch?v=fDb4nn4dQsE
優酷: https://v.youku.com/v_show/id_XNDU2OTM5Nzg4NA==.html
如有需求,我們也可向具有相關操作經驗的技術人員提供培訓。ERT-PCR核酸檢測方法由於其較高靈敏度,可以應用於諸如社區或隔離人員篩查和治愈病人出院前的核查。
Q: How is ERT-PCR technology operated and applied?
A: We have prepared operation manuals and video guides for the use of ERT-PCR technology. If required, we can provide training to technicians with the relevant operating experience. Due to its high sensitivity, ERT-PCR technology detection can be applied to the general community and medical teams involved in combatting coronavirus for early diagnosis.
Video guidance:
Youtube: https://www.youtube.com/watch?v=fDb4nn4dQsE
Youku: https://v.youku.com/v_show/id_XNDU2OTM5Nzg4NA==.html
另外,這次發佈會聯合黃乾亨基金、基因港(香港)生物科技有限公司、李氏大藥廠控股有限公司、依納康科技有限公司、晶準生物科技有限公司及海康生命科技有限公司,各家公司希望運用他們的資源,在新型冠狀病毒疫情下為社會出一分力,分別捐贈口罩、酒精搓手液和營養補充劑給有需要人士。
In addition, Hong Kong Biotechnology Organization was joined by other biotech and pharmaceutical companies: Philip K.H. Wong Foundation, GeneHarbor (Hong Kong) Technologies Limited, Lee’s Pharmaceutical Holdings Limited, eNano Health Limited, Cellomics International Limited and Hai Kang Life Corporation Limited, to provide resources against the new coronavirus epidemic by donating masks, alcohol hand rubs, and nutritional supplements to those in need.